CANINE TISSUE MICROARRAYS

Tissue microarrays provide the ability to evaluate the presence of a protein in multiple samples within a single experiment. The construction of an array begins by reviewing each tissue sample histologically within a parent paraffin block/section. A representative section, usually .6mm, is then removed from the tissue sample and placed in a new paraffin block. Once all desired tissue samples from a series of patients have been placed in the new block, multiple slides are cut. Each of the COP’s arrays has been reviewed histologically and clinical outcome data is available for most samples.

In collaboration with the NCI-CCR Tissue Array Research Program (TARP), Colorado State University, the University of Wisconsin at Madison, and the University of California at Davis, several canine tissue microarrays have been developed (see below).

Canine Osteosarcoma Array #1: Primary tumor from 75 distinct cases, 15 pulmonary metastases, and normal tissue.

Canine Lymphoma Array #1: Under Construction

Canine Osteosarcoma Array #2: Primary tumor from 145 distinct cases, 35 pulmonary metastases, and normal tissue.

Canine Nasal Carcinoma Array: Primary tumor from 36 distinct cases, and normal tissue.

Canine Validatoin Array: Primary tumor from 99 distinct cases, including 16 different histoliges, and normal tissues. This array will allow researchers to evaluate antibodies for cross-reactivity across dog, human, and mouse.

Canine Prostate Cancer Array #1: Under construction.

If you are interested in providing samples for an array, or would like to request slides, please contact Christina Mazcko at mazckoc@mail.nih.gov

For all general tissue microarray inquires, contact Christina Mazcko at mazckoc@mail.nih.gov

Frequently Asked Questions (FAQs)