Our Science – Bustin Website
Michael Bustin, Ph.D.
Chromosomal Proteins and Chromatin Function
Precise and specific interactions between chromosomal proteins and the chromatin fiber play a key role in epigenetic regulation, transcription, replication and DNA repair and therefore affect the orderly progression of biological processes such as development and differentiation. Numerous diseases, including cancer, are associated with changes in chromatin structure and function. The research aim of the Protein Section is to study the molecular mechanisms whereby nuclear proteins affect the structure and function of chromatin and play a role in establishing the cellular phenotype. The focus is on architectural proteins such as HMGN, the linker histone H1 and additional members of the high mobility group (HMG) protein family, which have been shown to affect the structure and function of chromatin and play a role in development and disease.
The laboratory employs a multidisciplinary approach, including analysis of transgenic and knock-out mice, and methodologies used in molecular biology, biochemistry, cell biology and immunochemistry, to study:
1. Molecular mechanisms whereby chromosomal proteins modulate gene expression from chromatin.
2. Mechanisms whereby HMGN chromosomal proteins affect the cellular phenotype and modulate embryonic differentiation.
3. The role of HMGN chromosomal proteins in modulating DNA repair processes.
4. The role of architectural chromatin binding proteins in epigenetic regulation.
5. Global organization of architectural proteins in the nucleus and in chromatin and their role in chromatin dynamics.
For more details about our research program, list of publications, and representative results please visit the Bustin Laboratory Web site.
This page was last updated on 2/19/2013.